NEWS
2020.06.26
(Vol.2)”Kaneka Easy DNA Extraction Kit version 2″ : Speeding up the detection of Phytophthora by the LAMP method.
Kaneka Easy DNA Extraction Kit version 2 has been published in the following paper.
＜Summary＞
Detection of the genus Phytophthora and the species Phytophthora nicotianae by LAMP with a QProbe
A. Hieno et al., The American Phytopathological Society, 287, 176–182 (2020)
In order to effectively quarantine fungi of the genus Phytophthora, A primer set for the LAMP method specific to the genus Phytophthora and P. nicotianae species-specific quenching probes ( (QProbe) was designed to establish a simultaneous detectable LAMP detection method.
The specificity of Phytophthora genus-specific primers was assessed for all 161 groups of species and we have confirmed that it is detectable by.
Kaneka Easy DNA Extraction Kit version 2, which can extract DNA from plant samples in a short time The simultaneous detection method was performed by extracting Phytophthora DNA using Phytophthora DNA was detected in all plant samples, confirming that P. nicotianae could be specifically identified. Phytophthora DNA was detected in all plant specimens, and it was confirmed that P. nicotianae could be specifically identified. This method of simultaneous detection allows faster and easier inspection at quarantine.
https://apsjournals.apsnet.org/doi/pdf/10.1094/PDIS-12-19-2523-RE
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