Kaneka’s “MyAbscope” has been published in the following paper.

• Kaneka’s “MyAbscope”

＜Paper information＞
Rapid DNA template preparation directly from a rice sample without purification for loop-mediated isothermal amplification (LAMP) of rice genes
J. Narushima et al., Bioscience, Biotechnology, and Biochemistry, 84, 670-677 (2020)

＜the Summary of the Paper＞
The authors have developed a pretreatment method that allows for rapid DNA templates from a single rice grain (Oryza sativa) or rice flour using the LAMP method.

The LAMP reaction was performed using DNA extracts obtained from alkali lysis（25 mM NaOH、0.2 mM EDTA） and neutralization reagents（40 mM Tris–HCl [pH 5]）. The results were comparable to the reaction efficiency of the LAMP reaction with the same amount of DNA template obtained by purification.
The DNA extracts obtained with the alkaline-degrading and neutralizing reagents described above have been verified to be stable enough to be stored at room temperature for up to 6 days.

This DNA extraction method, developed for the detection of rice genes by the LAMP method, makes it possible to detect rice genes quickly, easily, and at low cost without the use of special experimental equipment.
The authors developed the LAMP method, which allows visual (color change) detection of nucleic acid amplification for the detection of single rice grains and rice flour without laborious DNA purification or special laboratory procedures.

In this paper,Kaneka’s “MyAbscope” was used to perform the LAMP method, which enables this nucleic acid amplification to be detected visually (color tone change).

• Kaneka’s “MyAbscope”

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