This page provides answers to our survey by customers who have used Kaneka Easy DNA Extraction Kit version 2. They are published as they are.
This is not a guarantee of the accuracy or reliability of extraction results of the samples. Whether or not DNA can be extracted depends on the quantity, type and condition of each sample. If you wish to know whether this kit is suitable for your purpose, we recommend trying a free sample.

• I am repeatedly using it.
• We were able to extract DNA in a short time and get results efficiently.
• It was simple and easy and it saved time.
• We can perform genotyping efficiently because we can extract DNA easily and stably.
• We feel your product is excellent: it purifies DNA simply and easily at low cost. There was little variation in the amount of roughly purified DNA. We would like to continue to use it.
• Unfortunately, compared with the method we currently use, the kit did not produce a significant improvement in the result.
• We didn’t see any difference in genotyping between this kit and the conventional method of DNA extraction using NaOH, EDTA and HCL. Does it contain any substance that removes PCR inhibitors?
• We would like to continue to use it.
• It was fast and easy.
• The kit is good as it is quick.

• We were able to extract DNA from some mice but not from others. We found it a little inconvenient. It will be helpful if there is information on whether you can incubate for more than eight minutes or how long you can continue before it fails.

• Compared with DNA obtained using Proteinase K or phenol extraction, those by this kit resulted in faint or no bands in PCR after genotyping. We cannot use this kit for our mice.

• We used the extracted DNA samples for a digital PCR demonstration. It was very simple and easy, and we were able to detect what we aimed to.

• It was easier and quicker than proteinase K + column purification kit and the results were the same.

• We were able to extract DNA from four strains out of all the mice we are currently breeding. The process took a short time and that increased the research work efficiency. It was particularly useful when we wanted to make a diagnosis on the day.

• It is cost-effective.
• We were able to extract DNA from four strains out of all the mice we are currently breeding. The process took a short time and that increased the research work efficiency. It was particularly useful when we wanted to make a diagnosis on the day.

• We used PCR for students’ school projects. DNA was extracted easily. The kit was very helpful.

• It is better than other kits in terms of time, procedure and yield.

• We were having a hard time having to purify DNA every time we needed to check for foreign genes. Using this kit, we can prepare DNA quickly and detect foreign genes using PCR. We thus find it extremely handy.

• We were able to extract DNA quickly from 10 types of cloned T cells (about 5,000 - 10,000 cells) and confirm the genome sequences of nested PCR products.

• We were able to extract DNA with easy operation and use the extract as a PCR template.

• This is good as it is easy.

• The process is simple and we find it useful for processing a large amount of samples rapidly.

• It is simple and easy to use and suitable for research. Even students unfamiliar with the procedure could perform smoothly.

• DNA was extracted rapidly, which led to greater efficiency.

• We extracted DNA from the roots of lettuce infected with a pathogen and amplified DNA of the pathogen using real-time PCR.

• We compared with buffers we made. The results were similar.

• It is easy but the purity does not appear to be high.

• We were able to prepare DNA templates and perform PCR amplification easily using the kit together with micro-pestles.

• We extracted genomic DNA necessary for MIG-seq, one of the genotyping by sequencing methods, and had excellent amplification of the extracted DNA, which enhanced the efficiency of research.

• We were able to extract viral DNA from fish spleens but we would like to check whether they are parasites. The operation is extremely simple and easy, and thus it is convenient to do during busy daily work.
• A large amount of DNA was extracted. We diluted it for use. It was helpful as it reduced inspection time.

• It is as easy as even an elementary school student can do in a short time.

As the extraction steps were very simple, we were able to minimize the variation in DNA recovery rate between samples. Considering the simplicity of the procedure, the DNA recovery rates are surprisingly high.

• Genomic DNA usable as a PCR template was extracted easily. In alkaline lysis (50 mM NaOH) we usually use, usable types of PCR enzymes are limited. With this kit, we were able to perform amplification using versatile enzymes (except for inhibitors like melanin and polysaccharide. BSA is contained?).

• It was simple and easy without the need to use columns.

• We were able to extract DNA quickly but the quality was not good enough for the subsequent analysis.

• Yet to be determined

• We had hoped the kit would help us genotype genome-edited cells easily and efficiently but it did not with euglena.

• The operation was easy.

• Using extracted DNA as a template, we performed typing PCR with three primer sets but none of them went well: resulting in non-specific bands different from expected bands or no bands at all. Too many bacteria (1 x 106 cells/100 uL extraction kit) may have been used for extraction. However, we had a good result of the PCR above with DNA extracted in another method. We therefore haven’t optimized the conditions.
  As the DNA extraction procedure was easy, if we find the optimum conditions, we may use it in the future.

• We varied the number of bacterial cells between 1 x 107 and 5 x 108 and we were able to detect DNA from 1/5 of 5 x 107 using agarose gel stained by Midori Green Xtra. We were able to extract DNA without damage.

• We use the kit for simplified purification of genomic DNA of basidiomycetous yeast (to use as a template for PCR). Some fungi are not amplified by direct PCR from colonies in agar media but PCR works if DNA is extracted using this kit. There are no yeasts that we haven’t been able to extract DNA from.

• We would like to use it when we need to prepare many extracts.

• No data

• We were able to extract DNA more quickly than we did with the kit we usually used.

• The operation was simple and easy and DNA was extracted quickly. There was not much difference in the purity of DNA samples between this kit and other makers’ kits.

• Compared with the kits we used before, this kit required few steps of operation and extraction was fast and reliable.
  We have decided to continue to use this kit because we had a good result with the free sample and the cost was low.

• The simplicity and ease of operation are good. We would like to see if the subsequent experiment process goes well even if we use any type of enzyme.

• We tried a process of boiling in water, another kit, and Kaneka Easy DNA Extraction Kit version 2. The procedure was easy and even students were able to extract DNA stably.

• It didn’t work out well in my research because the samples required high sensitivity.