ProductKaneka Easy RNA Extraction Kit for RT-PCR
Kaneka Easy RNA Extraction Kit for RT-PCR
RNA extract applicable for RT-PCR can be obtained by simply adding the reagents to the sample (blood, cultured cells, etc.) and heating it. The operation requires about 30 minutes and since no organic solvent or special equipment is necessary, it shortens processing time and make extraction easier than the spin column method.
Applicable samples: Blood and cells
Application: Gene expression analysis (RT-PCR)
Extraction of RNA respectively from adherent cells (HEK293T) and floating cells (Jurkat), both of which had been prepared to have 105 cells, using this product. We then used the extract as a template RNA and performed RT-PCR targeting ACTB mRNA using T3000 Thermocycler (Biometra) with PrimeScript® One Step RT-PCR kit Ver. 2 (Takara Bio). We subjected the obtained RT-PCR product to electrophoresis and detected amplified fragments specific to the template RNA (Chart 1, 2). We also used the same extract as a template and performed PCR using T3000 Thermocycler (Biometra) with TaKaRa Ex Taq® (Takara Bio) in order to confirm that the extract did not contain any genomic DNA from the sample. We subjected the obtained PCR product to electrophoresis and confirmed that no amplified fragment was detected (Chart 1, 2).
Extraction of RNA from mouse blood anticoagulated with heparin using this product. We then used the extract as a template RNA and performed RT-PCR targeting H3f3a mRNA using T3000 Thermocycler (Biometra) with PrimeScript® One Step RT-PCR kit Ver. 2 (Takara Bio). We subjected the obtained RT-PCR product to electrophoresis and detected amplified fragments specific to the template RNA (Chart 3). We also used the same extract as a template and performed PCR using T3000 Thermocycler (Biometra) with TaKaRa Ex Taq® (Takara Bio) in order to confirm that the extract did not contain any genomic DNA from the sample. We subjected the obtained PCR product to electrophoresis and confirmed that no amplified fragment was detected (Chart 3).
KN-T110004
Kaneka Easy RNA Extraction Kit (for RT-PCR)
100
・Storage temperature: Reagent A (-20℃ to 4 ℃)
Frozen: DNase I (below -20℃)
・Expiration Date: 1 year from manufacturing