ProductKANEKA DNA Chromatography MABC/erm(41)
KANEKA DNA Chromatography MABC/erm(41)（For Research）
＜Feartures＞
- KANEKA DNA Chromatography MABC/erm (41) (research reagents) is intended to identify three subspecies of Mycobacteroides abscessus complex (MABC) (M. abscessus subsp. abscessus [M. abscessus], M. abscessus subsp. bolletii [M. bolletii], and M. abscessus subsp. massiliense [M. massiliense]) and two genetic types (T28C, full-length*1) related to erm (41) in bacterial suspension or bacterial broth medium based on the multiplex PCR and nucleic acid chromatography techniques.
*1. T28C strain: A strain in which nucleotide 28 of erm (41) is C. Full-length: A strain that keeps the full length of the erm (41) gene intact without any partial deletions (nucleotides 64–65 and 159–432 are typically deleted). KANEKA DNA Chromatography MABC/erm (41) was co-developed by Dr. Yoshihiko Hoshino, the National Institute of Infectious Diseases, and Kaneka Corporation. Its detection capability was published in EBioMedicine (2021 Feb; 64:103187). - Operation steps: culture → DNA extraction*2 → amplification (PCR) → chromatographic detection.
- Applicable specimens: Suspension or culture medium of cultures grown on solid or liquid medium.
・Liquid medium equivalent to McFarland No.1 (about 10^8cfu/mL) or liquid medium that is positive for culture.
・Bacterial suspension of colonies prepared in sterile distilled water to McFarland No.1 level.
– Results of examples – * Invalid if the control (C) line is not colored (illustrated example)
※2 It is recommended to use KANEKA Microbial DNA Extraction Reagent for the extraction of DNA.
Product Code
Product Name
Items
Units(Tests)
Product Code
KN-T10C815
Product Name
KANEKA DNA Chromatography MABC/erm(41)
Items
M. abscessus subsp. abscessus 【M. abscessus】
M. abscessus subsp. bolletii 【M. bolletii】
M. abscessus subsp. massiliense 【M. massiliense】
erm(41) full-length
erm(41) T28C
Units(Tests)
20
・Storage temperature: (2°C to 30°C) Test Strip, Detection Buffer, Results Card
（Freezing temperature (-20°C or lower): PCR Mix*3, Primer Mix
*3. Contains heat-resistant DNA Polymerase, dNTPs, dUTP and UNG (Uracil DNA Glycosylase).
・Expiry date: 1 year from date of manufacture